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Occult blood feces

Patient preparation

  1. Whenever possible, patient should be on meat-free (well-cooked pork, poultry and fish allowed), high-residue diet starting 2 days before and during test. Avoid peroxidase and peroxidase-like substances (horseradish and turnips) and preparations containing Vitamin C.
  2. Discontinue aspirin and nonsteroidal anti-inflammatory drugs for 7 days before and during the test. Acetaminophen (Tylenol) is allowed.


  1. Collect random specimen in a plastic container.
  2. Use applicator within Hemoccult test kit to pick up and immediately apply thin smear of fecal material inside Box A of the kit. Delay in application may cause false negatives to occur.
  3. Reuse applicator to pick up 2nd portion of fecal material from different part of feces.
  4. Apply thin smear within Box B. Close kit cover.
  5. Collect additional or repeat specimens at least 12 hours apart.
  6. Ideal collection: 3 specimens from 3 separate bowel movements on consecutive days.

Storage instructions

  1. Maintain prepared smears at room temperature.
  2. Shield from heat, light, and volatiles.

Causes for rejection

  1. Contamination with urine or barium.
  2. Expired collection kit.
  3. Smears received more than 14 days after collection.

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